Analysis of Gene Expression Profiles of Sterile and Fertile Anthers of Thermo-sensitive Genic Male Sterile Wheat(Triticum aestivum L.)

To reveal the molecular mechanism of the temperature/photoperiod sensitive male sterile lines of wheat(Triticum aestivum L.), the differently expressed genes in anthers at the tetrad and bicellular stage of the sterile and fertile lines of Bainong sterility(BNS) were analyzed using Affymetrix gene chip technology of wheat. The semi- quantitative RT- PCR was utilized to characterize some sterile candidate genes. The results showed that among the 61 127 probes transcripts, there were 418 valid transcripts presenting different expression between the sterile and fertile lines. Among these transcripts, 142 of them were down- regulated and 276 of them were up-regulated in the bicellular stage. Functional classification indicated that these genes participated in a series of significant life processes of metabolism process, cytological process, biological regulation and irritative reaction etc. Moreover, to verify chip data, 10 genes of leucine- rich repeats(Ta.17228.1), ATP- binding cassette transporter(Ta.21809.1), peptidoglycan binding protein(Ta.22570.1),Ta.37113.2(unknown), cytochrome P450(Ta.19531.1), phosphoribulose kinase(Ta.18368.1), transcriptional regulator(Ta.9239.1), peptidase C1 A subfamily(Ta.37113.1), thionin super family(Ta.21983.1) and V- type proton ATPase subunit D(Ta.18091.1), differentially expressed significantly, were identified by c DNA semiquantitative RT- PCR. Result showed that the expression models of the 10 genes were concordant with the result of gene chip detection. Conservative structure domain analysis showed that Ta.18091.1, Ta.21809.1and Ta.19531.1 most likely involved in the abortive anthers in the BNS sterile lines. The results provide important theoretical basis for sterility- related genes cloning and verification, and contribute to find the key gene which leads to pollen abortion in thermo-sensitive genic male sterile wheat(BNS).