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[The effects of ecdysterone on the apoptosis and necrosis of cultured HUVEC induced by endotoxin].

Shi F, J Zhang, M Zhang

Abstract

OBJECTIVE: To study the protective effects of ecdysterone (EDS) on the apoptosis and necrosis of cultured HUVEC induced by endotoxin. METHODS: HUVEC was cultured in vitro. Apoptosis and necrosis of HUVEC were induced by LPS, and EDS was added in test specimen. Apoptosis was determined by means of flow cytometry and fluorescent microscopy. RESULTS: There was relatively less number of apoptosis and necrosis of in vitro cultured normal HUVEC than that after LPS stimulation, whereas apoptosis dominated over necrosis. After EDS was added to the culture of HUVEC, cell apoptosis and necrosis decreased simultaneously. CONCLUSION: EDS might exert protective effects on cultured HUVEC against apoptosis and necrosis induced by LPS.

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Key concepts: Apoptosis, Necrosis, Flow cytometry, Tumor necrosis factor alpha, In vitro, Molecular biology, Biology, Chemistry