Interaction of Fluorine,Calcium and Iodine on Thyroid Index and Morphosis in Rats

Objective To study the interaction of fluorine,calcium and iodine on thyroid weight and morphological structure in rats.Methods Eight Wistar rats were randomly divided into 8 groups: moderate concentrations of fluorine(90 μg/d),calcium(13 mg/d) and iodine(3.5 μg/d);moderate concentrations of fluorine,iodine and higher concentration of calcium(260 mg/d);moderate concentrations of calcium,iodine and higher concentration of fluorine(2 700 μg/d);moderate concentration of iodine and higher concentrations of fluorine,calcium;moderate concentrations of fluorine,calcium and lower concentration of iodine(0.23 μg/d);moderate concentration of fluorine,higher concentration of calcium and lower concentration of iodine;moderate concentration of calcium,higher concentration of fluorine and lower concentration of iodine;higher concentrations of fluorine,calcium and lower concentration of iodine based on 2×2×2 factorial design.After six months of treatment,thyroid index was measured and pathological examination was conducted.Results Thyroid weights of male rats in lower iodine group was higher than that in moderate iodine group.Significant interaction effects of fluorine and calcium,fluorine and iodine on thyroid index of female rats were found.Significant interaction effects of fluorine and calcium on thyroid coefficient in female rats were found,and the synergy in moderate iodine,antagonism in lower iodine was seen.The interaction effects of combined fluorine with iodine showed an antagonism in female rat thyroid.The pathological examination showed a similar result to that in thyroid index calculation.Conclusion Low iodine intake may increase thyroid index in male and female rats,high fluorine exposure may decrease thyroid index,increase thyroid cell and decrease thyroid colloid.