Interaction of fluorine,calcium and iodine on thyroid weight and function of rats

Objective To study the interaction of fluorine,calcium and iodine on thyroid weight,TSH,TT3 and TT4 of rats.Methods Wistar rats were randomly divided into 8 groups: moderate concentrations of fluorine,calcium and iodine;moderate concentrations of fluorine,iodine and higher concentration of calcium;moderate concentrations of calcium,iodine and higher concentration of fluorine;moderate concentration of iodine and higher concentrations of fluorine,calcium;moderate concentrations of fluorine,calcium and lower concentration of iodine;moderate concentration of fluorine,higher concentration of calcium and lower concentration of iodine;moderate concentration of calcium,higher concentration of fluorine and lower concentration of iodine;higher concentrations of fluorine,calcium and lower concentration of iodine based on 2×2×2 factorial design.After six months,thyroid weights were measured.Results According to the results of factorial ANOVA,thyroid weights and TSH of rats in lower concentration of iodine group is higher than those in moderate concentration of iodine groulp.Significant interaction effects of fluorine with iodine on TT3 of rats were found.TT3 of rats in lower concentration of iodine group is higher than that in moderate concentration of iodine group,while certain fluorine and calcium concentrations were moderate.TT3 in higher concentration of fluorine group is higher than that in moderate concentration of fluorine group,while certain iodine and calcium concentration were moderate.TT4 of rats in lower concentration of iodine group is lower than that in moderate concentration of iodine group.No effect were found in other groups.Conclusions Iodine failure may change thyroid weight and relative function of rats.There are interaction effects of fluorine with iodine on TT3 of rats.Fluorine could affect function of thyroid directly or indirectly.