2009•Zhonghua zhongyiyao xuekanRequires access

Canparison of Seven Ginsenoside Contents in Shengshaishen Hongshen and Linxiashen

Deqiang Dou

Abstract

Objective:A HPLC method for the simultaneous determination of ginsenoside-Rg1,-Re,-Rf,-Rb1,-Rc,-Rb2 and Rd was established for the comparison of the ginsenoside contents in Shengshaishen,Hongshen and Linxiashen from Benxi.Methods:HPLC with Diamonsil C18(250mm×4.6mm,5μm)column was used,the 50% acetonitrile-water(gradient elution)as a mobile phase with the column temperature at 30℃ and the flow rate 1.0mL·min-1.The UV wavelength used for detection was set at 203 nm and the analysis time was 100 min.Results:This method can be used for the simultaneous determination of 7 ginsenosides in different types of ginseng.Conclusion:the contents of ginsenosides in the three kinds of ginseng showed obviously difference.

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Key concepts: Gradient elution, Chromatography, Ginsenoside, Ginseng, Ginsenoside Rg1, Chemistry, High-performance liquid chromatography, Medicine