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2007•Strait Pharmaceutical JournalRequires access

Content determination of ginsenoside Rg1 and Rb1 in Renax Oral liquids by HPLC

Xiaolan Liu

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Abstract

OBJECTIVE A method for determination of ginsenoside Rg1 and Rb1 in Renax oral liquids by HPLC was created.METHODS The HPLC methods used a Waters XTerraTM RP18 column,with mobile phase A (acetonitrile) and mobile phase B (water) gradient elution.The column temperature was 25℃,the wavelength for detection was 203nm,the flow rate was 1.0mL·min-1.RESULTS Ginsenoside Rb1 and Rg1 showed good linear relationship at the range of 0.4～4.8 μg,the average recovery of Rb1 and Ginsenoside Rg1 were 97.9% (RSD=2.05%) and 99.1%(RSD=1.96%)respectivvely.CONCLUSION The result was accurate and the reproducibility was good.The method can be used for quality control of tinctura.

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OBJECTIVE A method for determination of ginsenoside Rg1 and Rb1 in Renax oral liquids by HPLC was created.METHODS The HPLC methods used a Waters XTerraTM RP18 column,with mobile phase A (acetonitrile) and mobile phase B (water) gradient elution.The column temperature was 25℃,the wavelength for detection was 203nm,the flow rate was 1.0mL·min-1.RESULTS Ginsenoside Rb1 and Rg1 showed good linear relationship at the range of 0.4～4.8 μg,the average recovery of Rb1 and Ginsenoside Rg1 were 97.9% (RSD=2.05%) and 99.1%(RSD=1.96%)respectivvely.CONCLUSION The result was accurate and the reproducibility was good.The method can be used for quality control of tinctura.

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Available abstract

OBJECTIVE A method for determination of ginsenoside Rg1 and Rb1 in Renax oral liquids by HPLC was created.METHODS The HPLC methods used a Waters XTerraTM RP18 column,with mobile phase A (acetonitrile) and mobile phase B (water) gradient elution.The column temperature was 25℃,the wavelength for detection was 203nm,the flow rate was 1.0mL·min-1.RESULTS Ginsenoside Rb1 and Rg1 showed good linear relationship at the range of 0.4～4.8 μg,the average recovery of Rb1 and Ginsenoside Rg1 were 97.9% (RSD=2.05%) and 99.1%(RSD=1.96%)respectivvely.CONCLUSION The result was accurate and the reproducibility was good.The method can be used for quality control of tinctura.

Key concepts: Ginsenoside Rg1, Chromatography, Gradient elution, High-performance liquid chromatography, Chemistry, Linear range, Ginsenoside, Linear relationship

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