Protective effects of compound injection of the Danshen on vascular endothelial cells induced by hypoxia-reoxygenation injury

OBJECTIVE To study the protective effects of the compound injection of Danshen on cultured vascular endothelial cells VECs induced by hypoxia-reoxygenation injury. METHODS By using primary culture of human umbilical vein endothelial cells HUVECs in vitro,the VECs were divided into three groups,the morphological changes of VECs in each group were observed by the inverted microscope,the optical density OD value of cells was detected by MTT colorimetric method,and nitric oxide NO in the supernatant fluid of cultured cells was detected by spectrophotometer. RESULTS MTT assay showed that the Danshen had the protective effects for VECs induced by hypoxia-reoxygenation injury in 0.25～12.50 g/L of concentration.The OD value was obviously decreased in the hypoxia-reoxygenation group,but one was increased in the normal and the Danshen groups and was highest at 12.50 g/L of the Danshen concentration.The OD value had significant difference between normal group, Danshen group and hypoxia-reoxygenation group.The content of NO in the supernatant fluid of cultured cells also had significant different between the hypoxia-reoxygenation group,normal group and Danshen group.NO content was decreased in the hypoxia-reoxygenation group,and that was increased in the normal and Danshen groups. CONCLUSION The compound injection of Danshen has protective effects for the HUVEC induced by hypoxia-reoxygenation injury and anti-anoxic injury by the mechanisms of abating the lipid peroxidation of cell,counterpoising the content of NO.