Protective effect of Danshen injection in high concentration on endothelial cells injured by high glucose

[Objective] To explore the effect of Danshen injection on human vascular endothelial cells injured by high glucose. [Methods] The endothelial cells were obtained by perfusion digestion of blood from umbilical vein using type I collagenase(0.1%), and the second passage of endothelial cells was incubated in 96 plate. The formed subconfluent, endothelial cells were incubated with different medium respectively: normal control, group inured with high glucose and protected group with different concentration of Danshen injection. 48 hours later, the number of survived cells were determined with MTT. [Results] At the concentration of 10-1,10-2,10-3mg/ml, Danshen injection could improve the protective effect against the injury of endothelial cells harmed by high glucose(P0.05), at the concentration of 10-4mg/ml could not improve the protective effect(P0.05). [Conclusion] Danshen injection of high concentration can protect endothelial cells harmed by high glucose.