Protective Effects of the Lyophiled Powder Injection of Bear Bile on Vascular Endothelial Cells Induced by Hypoxia-reoxygenation Injury

ObjectiveTo study the protective effects of the lyophiled powder injection of bear bile on cultured vascular epithelial cells(VEC) induced by hypoxia-reoxygenation injury,and study the mechanisms of anti-atherosclerosis.MethodsBy using primary culture of human umbilical vein endothelial cells(HUVEC) in vitro, the VECs were divided into three groups.The morphological changes of VEC by the inverted microscope,the optical density(OD) value of cells were detected by MTT colorimetric method,content of MDA and NO in the supernatant fluid of cultured cells were detected by spectrophotometry.ResultsThe MTT assay showed that OD value had significant difference(P0.01) between normal group,bear bile group and hypoxia-reoxygenation group.The MDA content increased and NO content decreased in the hypoxia-reoxygenation group,in contrast,MDA content decreased and NO content increased in the normal and bear bile groups.ConclusionThe results indicate that the lyophiled powder injection of bear bile has protective effects for the HUVEC injury induced by hypoxia-reoxygenation injury.