THE THERAPEUTIC EFFECT OF DEXAMETHASONE ON LIVER INJURY RATINDUCED BY LPS/D-GALN

The purpose of this article is to study the therapeutic effect of dexamethasone(dexamethasone,Dex) on lipopolysaccharide(LPS) combined D-galactosamine(D-GalN)-induced acute liver injury in rats and the corresponding mechanisms.Methods:(1)Rats were randomly divided into normal control group,model group,treatment group.Dex(10mg/kg),LPS(50g/kg) and D-GalN(300mg/kg) were injected intraperitonealy.,plasma alanine aminotransferase(ALT),aspartate aminotransferase(AST),and tumor necrosis factor-а(TNF-а) levels of liver tissues,and hepatic histopathological changes were examined 16h after the treatment;(2)Rats were randomly divided into normal saline group and dexamethasone group,the survival rate in rats was observed for 10d after treatment,and survival curves were made accordingly.Results: ALT,AST,TNF-а expression in model group were significantly higher than the control group.However,the expression of ALT,AST and TNF-а was decreased significantly in the treatment group compared with the saline group.the mortality rate of the dexamethasone group significantly reduced compared with the saline group.Conclusion;Dex protect rats from LPS / D-GalN-induced liver injury by down regulating the expression of TNFa.