The protective effect of Rosiglitazone on mice acute hepatic failure induced by D-GalN and LPS

Objective To observe the protective effect of Rosiglitazone on the mice acute hepatic failure induced by D-GalN and LPS,and to explore the possible mechanism.Methods Male mice were randomly divided into three groups: normal group,control group and therapy group.The control group and the therapy group were intraperitoneally injected by D-GalN/LPS,while the normal group was injected by saline;the therapy group was intragastircally lavaged by Rosiglitazone at 2 hours before the D-GalN/LPS injection,while the control group and the normal group were lavaged by saline. The 24 hours survival rate,the serum levels of ALT and AST,the histopathological changes of the liver and the levels of TNF-α and Caspase-3 mRNA of the liver were detected in each group.Results The 24 hours survival rate of the therapy group was significantly higher than that in the control group(P0.05).The serum levels of ALT and AST in the therapy group were significantly lower than those in the control group(P0.05).The degree of liver injury in the therapy group significantly reduced compared with control group.The levels of TNF-α and Caspase3 mRNA in the hepatic tissue of the therapy group were lower significantly than those in the control group(P0.05).Conclusion Rosiglitazone plays an effectively protective role in the acute hepatic failure of mice.Rosiglitazone can inhibit the inflammation of liver,decrease the necrosis and decrease the mortality of the acute hepatic failure.The possible mechanism is that rosiglitazone reduces the expressions of TNF-α and Caspase-3.