Experimental study on the effect of selective COX-2 inhibitor sinomenine on Hela cells

Objective: To study the inhibitory effect of a selective cyclooxygenase-2 inhibitor Sinomenine(SIN on Hela cells and further investigate the mechanism by which Sinomenine inhibited proliferation of Hela cells,and to afford laboratory data for application of SIN in the prevention and treatment for cervical cancer.Methods: Hela cells were treated with various concentration of SIN for 24,48 and 72h,the cell proliferation was estimated using the MTT assay,and the cell cycle distribution and apoptosis were examined by Flow cytometry.Results: SIN inhibits proliferation of Hela cells in a dose and time-dependent manner.Flow cytometry results demonstrated that the cell cycle was redistributed: the G1-Phase cell fraction was increased,while the S-Phase cell fraction was significantly decreased after the cells were treated with SIN(p0.05);SIN could induce apoptosis of Hela cells in a dose and time-dependent manner.Conclusion: SIN could inhibit the growth of Hela cells,the mechanism may relate to its blockage of cell cycle and apoptosis induction.