The Synergistic Antiproliferative Effect of Sinomenine and Carboplatin on Cervical Cancer Hela Cells

Objective:To study the effect and mechanism of selective cyclooxygenase-2 (COX-2) inhibitor sinomenine(SIN)on cervical cancer Hela cells,and to explore its synergistic effect with carboplatin on cervical cancer cells.Methods:MTT assay was used to determine the anti-proliferative effect of selective COX-2 inhibitor SIN and its combination with carboplatin on cervical cancer Hela cells. The cell cycle distribution and apoptosis were detected by Flow cytometry.Results:MTT assays showed that SIN inhibited the growth of cervical cancer Hela cells by concentration and time dependence,and synergistic effect appeared when combined with carboplatin. Flow cytometry results demonstrated that the cell cycle was redistributed:the G1-Phase cell fraction was conspicuous increased while the S-Phase cell fraction was significantly decreased after treated with SIN(P0.05). However,the result was opposite after treated with carboplatin. The apoptotic index was increased in SIN group than that in control group,which with time and dose dependence. The apoptotic index was significantly increased after treated with SIN combined with carboplatin.Conclusions:SIN inhibits the growth of cervical cancer cells by concentration and time dependence,the mechanism of which may relate to its cell cycle distribution and apoptosis induction. SIN combined with carboplatin could exert synergistic antiproliferative effect on cervical cancer Hela cells by influencing cell cycle distribution and apoptosis.