Construction and expression of prokaryotic expression vector for VP1 unique region gene segment of human parvovirus B19

Objective:To clone human parvovirus B19 VP1 gene.,construct the prokaryotic expression vector,express its product Methods:VP1gene was amplified by PCR and cloned into pGEM-T-easy plasmid,.After PCR selecting.it was subcloned into pGEX-4T-1 plasmid.The recombinant expressive vector of VP1 gene was transformed into E.coli BL21,and induced to express by IPTG in BL21.Then Western blotting identified the expression product .Results:Human parvovirus B19 VP1gene was coloned.Recombinant expression plasmid pGEX-4T-1 was constructed.The expressed fussion protein was confirmed by PAGE,This product was also proved to be the target protein via Western blotting.Conclusion:The human parvovirus B19 VP1gene and its prokaryotic expression products were obtained.It might be important for study on the function of human parvovirus B19VP1 and preparing the monoclonal antibody against human parvovirus B19 VP1.