Cloning and prokaryotic expression of VP2 gene from porcine parvovirus PPV-SD1 strain

In order to study the structure and function of VP2 protein from porcine parvovirus PPV-SD1 strain,full-length VP2 gene was amplified by PCR,and then cloned into pMD18-T and sequenced.The recombinant plasmid pET30a-VP2 was constructed by subcloning into expression vector pET30a(+) and transformed into RosettaTM.VP2 gene was successfully expressed in host cells when induced at 37 ℃ with 1.0 mM IPTG for 3-4 h.This recombinant protein with a molecular weight of about 68 ku was approved to have immunological activity by SDS-PAGE and Western-blotting.