Clone and expression of VP1 unique region gene segment of Chinese strain of human parvovirus B19

Objective: To construct the recombinant expressive vector of VP1 gene of Chinese strain of human parvovirus B19,and to obtain the expression of the structural protein VP1. Methods:VP1 gene segment was obtained from the recombinant clone vector of VP1 gene kept in our laboratory and sub cloned into expressive vector pQE 30. The recombinant expressive vector of VP1 gene was constructed and transformed into E. coli M15. IPTG was used to induce the expression of protein. Different conditions were taken and SDS PAGE was used to seek out the best way in which the protein was expressed. Results: Recombinant expressive plasmid VP1 pQE 30 was constructed. 15% SDS PAGE showed the molecular weight of the expressed fusion protein was about 22 000. The protein could be expressed under 25℃,but not under 37℃. Different concentrations of IPTG, from 0.05 mmol/L to 1 mmol/L, could induce the expression of the protein and the expressive ratio was similar, which was about (16±1)%. Conclusion: The prokaryotic expressive vector and the fusion protein of human parvovirus B19 VP1 gene of Chinese strain were obtained. It is important in the further purification of human parvovirus B19 VP1 protein and the preparation of the monoclonal antibody against VP1 structural protein and the B19 vaccine.