Effect of microcytes/schistocytes on platelet count when deficiency of portion platelet parameters measured by XS1000i Hematology analyzer

Objective To investigate the effect of microcytes/schistocytes on platelet count when deficiency of portion platelet parameters measured by XS1000i hematology analyzer. Methods After analyzed by XS1000i hematology analyzer, the samples which have MCV80fl and loss partial platelet parameters were re-measured with the PENTRA120Retic hematology analyzer, microscopic platelet count and,Wright staining for platelets. Results When PLT100×109/L, there were statistically significant differences in the platelet counts among XS1000i, PENTRA120Retic and microscopic counting(P0.01); when PLT100×109/L, there were no statistically significant differences in the platelet counts among XS1000i PENTRA120Retic and microscopic counting(P 0.01). When MCV was 70~80fl, 60~70fl and 60fl, respectively, the platelet counts of XS1000i were higher than those of microscopic counting, the difference was statistically significant(P0.01). The results of microscope examination were as follows: all 101 specimens were detected and had microcytes or(and) schistocytes, of which 60 cases were accompanied with large platelets and/or giant platelets, and 4 cases were accompanied with large platelets and platelet aggregation. Conclusions The samples with MCV 80fl and missing partial platelet parameters when analyzed by impedance hematology analyzer should be re-measured with the reference method recommended by ICSH or microscopic platelet counting, meanwhile, detect the morphology, number and distribution of abnormal blood cells by staining and microscopic examination.