Comparative Study on the Characteristic Chromatogram of the Crude and Processed Radix Paeoniae Alba from Hangzhou

OBJECTIVE To establish a sensitive and specific HPLC method for quality control of Radix Paeoniae Alba.METHODS HPLC method was applied for quality assessment of Radix Paeoniae Alba.HPLC analysis was performed on a Hanbon C18 column(250 mm×4.6 mm,5μm).The mobile phase consisted of acetonitrile(solvent B) and water containing 0.1%(v/v) phosphoric acid(solvent A) at a constant flow rate of 1.0mL/min.An increasing linear gradient(v/v) of solvent B was used.The injection volume was 20μL.The column temperature was set at 25℃.The chromatograms were monitored at 230nm.RESULTS The HPLC characteristic chromatogram of Radix Paeoniae Alba,showing 9 characteristic peaks,was established from its prepared samples of Radix Paeoniae Alba.CONCLUSION The characteristic chromatogram of Radix Paeoniae Alba with high specificity can be used to control its quality and assure lot-to-lot consistency.