2010•Chinese Journal of Spectroscopy LaboratoryRequires access
Study of the Content of Paeoniflorin and HPLC Fingerprint in Radix Paeoniae Alba
Wen Liu
Open publisher page 0 citations
Abstract
TitleStudy of the Content of Paeoniflorin and HPLC Fingerprint in Radix Paeoniae Alba
AuthorLIU Wen-Bin HE Zhen-Hui BIN Man-Rong(No.1 Substation of Guangzhou Institute for Drug Control,Guangzhou 510130,P.R.China)
A HPLC-fingerprint of Radix Paeoniae Alba was established and the drug's quality was evaluated by determining the content of paeoniflorin.Analysis was performed using a Hypersil ODS C18 column(4.6mm×250mm,5μm) with the mixture of acetonitrile-0.08% phosphoric acid solution as mobile phase in gradient mode,and the column temperature was at 30℃.Seventeen samples of the crude drugs of Radix Paeoniae Alba were determined.The mutual model was established and the similarity degrees were calculated.Similarity degrees of 8 samples were between 0.902—0.985 respectively.The limit of similarity degree for Radix Paeoniae Alba was set at 0.90.If the similarity degree was greater than 0.90,it was qualified crude drug,otherwise it was unqualified.The evaluation results from this method were the same as the results from the method published in the Chinese Pharmacopoeia(2010ed.) and it can be used to control the sulfide contended in Radix Paeoniae Alba.
KeywordsRadix Paeoniae Alba;HPLC;Fingerprint;Sulfide