Cloning and Character Analysis of A Novel Testis-specific Gene,TSF22,in Mice

Objective To identify genes that involved in spermatogenesis.Methods In order to screen the testis-specific genes,testes cDNA samples from BALB/c mice of different postnatal days(days 4,9,18,35,54 and 6 months)were performed with mouse whole genome Affymetrix chip.The characteristics of the selected gene were analyzed by various bioinformatic tools.The expression profile of the selected gene was identified by RT-PCR.Results By analyzing the hybridization signals,a gene with a differential expression in the developmental stages of testis was identified.This gene was designated as TSF22.The full length cDNA of 1 597 bp contained an open reading frame of 570 bp which encoded a putative protein of 190 amino acids and a molecular weight of 22.106 kD.RT-PCR analysis revealed that TSF22 mRNA was exclusively expressed in mice testis.Conclusions TSF22,functions as a testis-specific transcription factor,may play im-portant roles during spermatogenesis.