Optimization of Freeze-drying Process for Preparation of Oxaliplatin Loaded Nanostructured Lipid Carriers for Injection

Objective To investigate the freeze-drying formulations of oxaliplatin in oxaliplatin-loaded nanostuctured lipid carriers(OP-NLC) for injection.Methods The optimum freeze-drying preparation and techniques were established by single factor experiments and the characters were evaluated.Results The optimal freeze-drying technology was determined with 5% of mannitol as protective agent.No significant changes were found in the mean particle size,Zeta potential and EE%,which were(124±16) nm versus(115±17) nm,-10.8 mV versus-16.0 mV,and 67.3% versus 72.6% post-and pre-freeze-drying,respectively.Conclusion The stable freeze-drying preparation of OP-NLC could be prepared under the optimal condition.