Construction of a Homologous Recombination Plasmid Vector pMD-Fib-IE-EGFP Carrying EGFP as Reportor Gene

A new plasmid vector pMD-Fib-IE-EGFP which contains enhanced green fluorescent protein gene as reportor gene was constructed by subtitute EGFP for GFP in the original plasmid pG350. The recombinant plasmid was identified by restriction endonuclease enzyme analysis and PCR. The recombined plasmid was transferred to stomach cancer cell mediated by lipofectine. The transfected cell expressed strong fluorescence. The constructed plasmid vector provided foreign DNA material for transgenic silkworm.