Study on the Discrimination of Male Sterile Cytoplasm Types M_(5P) and Y_(II-1) with Leaf and Seed DNA in Maize

It had been reported some genes in mitochondria genome, such as T-urf13, atp6-C, orf355 and so on, are controlled and regulated the pollen sterility. Based on the published sequences of the above three genes fragments, three pairs of specific primers were elaborately designed for discriminating of new male sterile cytoplasm types M5P and YⅡ-1. Three sets of male sterile lines, whose nuclear background were different, were adopted in experiment. Their nuclear background were B77, U8112 and 150, respectively. Their cytoplasm types were T, S, C, M5P and YⅡ-1, genomic DNA extracted from the leaf and the seed as well as mitochondria DNA(mtDNA) were used as template in PCR reaction. The results showed: ①The PCR products used YⅡ-1 and M5P type male sterile lines gnomic DNA or mtDNA as temple were the same as those of C type. On the contrary, they were different from T and S group. It may be concluded that YⅡ-1 and M5P type could belong to C group. ②The results of PCR amplification used genomic DNA and mtDNA as template were the same, it indicated that it only need genomic DNA used as PCR reaction template for discriminating of male sterile cytoplasm types. ③This PCR based male sterile cytoplasm types discrimination strategy is not affected by the nuclear background of experimental materials.