Study on quality standard for Xiongyin Zhongfeng granules

[Objective] To establish the quality standard for Xiongyin Zhongfeng granules.[Methods] A identification of Xiongyin Zhongfeng Granules was performed by TLC.The ferulic acid and paeoniflorin were determinated by HPLC.Ferulic acid: It was separated on C18(250 mm×4.6 mm,5μm)column.The mobile phase was methanol-1% acetic acid solution(35∶65).The flow rate was 1mL/min.The detection wavelength was 321nm.Paeoniflorin: It was separated on C18(250 mm×4.6 mm,5 μm)column.The mobile phase was acetonitrile-0.01% phosphate solution(15∶85).The flow rate was 1 mL/min.The detection wavelength was 230 nm.[Results] Identification of ligusticum chuanxiong hort,angelica,pheretima,paeonia of Xiongyin Zhongfeng granules was established by TLC.The linear range of ferulic acid was 4.286~42.86 μg/mL(r=1.000).The average recovery was 99.7% with RSD=2.8%(n=6).The linear range of paeoniflorin was 0.058 72~0.587 2 mg/mL(r=0.999 99).The average recovery was 99.5% with RSD=0.67%(n=6).[Conclusion] These methods are simple,accurate and repeatable.They can be used as quality standard for Xiongyin Zhongfeng granules.