2012•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Chlorogenic Acid and Baicalin in Kunlun Chrysanthemum by HPLC

Zhi Ling

Abstract

Objective:To establish a HPLC method for the determination chlorogenic acid and baicalin in Kunlun Chrysanthemum.Method:The HPLC method was used.The sample was analyzed on an Inertsil ODS-SP(4.6 mm × 150 mm,5 μm) column at 25 temperature,with mobile phase consisting of methanol and 0.05% phosphoric acid(pH 3.0) by gradient eluteion at the flow rate of 0.6 mL.min-1,The wavelength was at 330 nm.Result:The regression equation of chlorogenic acid was linear in the range of 0.45-15.00 mg.L-1,A =55 102C-24 808,r = 0.999 8(n = 6).The average recovery was 102.58%(RSD 0.93%).The regression equation of baicalin was linear in the range of 0.90-30.00 mg.L-1,A = 25 599C-16 099,r = 0.999 7(n = 6).The average recovery was 104.90%,(RSD 1.12%).Conclusion:The method was stable and accurate,it is suitable to determine chlorogenic acid and baicalin in Kunlun Chrysanthemum

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Key concepts: Baicalin, Chlorogenic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship