Determination of Chlorogenic Acid and Baicalin in Fufangqinlan Oral Solution by HPLC

Objective To determine the contents of chlorogenic acid and Baicalin in fufangqinlan oral solution.Methods HPLC was performed by using C-l8 column.The chlorogenic acid were separated with methanol-0.4% phosphoric acid solution(24∶76)as a mobile phase and detected at 327 nm.The baicalin were separated with methanol-water-phosphoric acid solution(47∶53∶0.2) as a mobile phase and detected at 274 nm.Results The chlorogenic acid had good linear relationship(r=1.000 0) within the range of 0.118~1.18 μg and the average recovery was 98.5% with RSD of 1.8 %.The baicalin had good linear relationship(r=0.999 8) within the range of 0.069 6~0.556 8 μg and the average recovery was 98.2% with RSD of 1.4%.Conclusion The method is simple,accurate and reprodueible.It can be used for guality control of Fufangqinlan oral solution.