Genetic Polymorphism of TLR4 at Intronic Region in Guangxi Population

Objective To investigate the distribution of single nucleotide polymorphism( SNP) of Toll-like receptor4( TLR4) gene in Guangxi population,and to discuss the difference among various groups. Methods Polymerase chain reaction-single base extension( PCR-SBE) and DNA sequencing method were used to analyze the rs11536879A / G polymorphism of TLR4 gene at the intronic region among 196 healthy cases in Guangxi( 115 male cases,81 female cases)which was compared with the data of other 4 populations( Hapmap-CEU,Hapmap-HCB,Hapmap-JPT,Hapmap-YRI)from the Human Genome Project group( Hapmap). Results There were genotypes of AA,GA,GG in rs11536879A / G of TLR4 gene at the intronic region in Guangxi population,the frequencies of genotype and allele distribution of rs11536879A / G polymorphisms showed no significant difference between female and male groups( P 0. 05). Guangxi population showed a significant difference in the frequencies of genotype and allele distribution of rs11536879A / G polymorphism in contrast with Hapmap-CEU,Hapmap-JPT and Hapmap-YRI( P 0. 05),but showed no significant difference in contast with Hapmap-HCB( P 0. 05). Conclusion The frequencies of genotype and allele distribution of genetic polymorphisms at the TLR4 intronic region in Guangxi population show no significant difference between the male and the female,but show a significant difference among different regions and races.