Genetic Polymorphism of IL-18 Gene Promoter Region in the West of Guangxi Zhuang Populations in China

Objective:To study the frequencies of allele and genotype distribution of interleukin-18(IL-18)gene promoter region single nucleotide polymorphism(SNP)in the west of guangxi zhuang populations in china,and to analyze the distributions of IL-18polymorphism among different races.Method:The IL-18gene promoter region rs360722G/A polymorphism was examined by the polymerase chain reaction-single base extension(PCR-SBE)technique and DNA sequencing methods in 150the west of guangxi zhuang populations,frequencies of allele and genotype of IL-18gene rs360722G/A polymorphism were analyzed for the west of guangxi zhuang populations compared with other the four populations(HapMap-HCB,HapMap-YRI,HapMap-CEU and HapMap-JPT)from human genome project group(Hapmap)data.Results:IL-18genotype frequencies of GG,GA and AA were 39.3%,48.7%and 12.0%,and allele frequencies of G and A were 63.7%and 36.3%in the west of guangxi zhuang populations respectively.The frequencies of genotype of IL-18gene rs360722G/A polymorphism were not significant difference between male and female group(P0.05).The frequencies of allele and genotype distribution of IL-18gene rs360722G/A polymorphism were significant difference compared with HapMap-CEU,HapMap-YRI and HapMap-HCB populations(P0.05).Conclusion:There are IL-18gene polymorphism in the west of guangxi zhuang populations,the frequencies of polymorphism distribution of IL-18are significantly difference compared with others ethnic populations.It might be one of the reasons why the clinical spectrunm of some IL-18related diseases is different among different races.