Research on the extraction technology of total alkaloid from konjac processing residues

In order to increase the additional value,reduce the processing cost and solve the pollution of konjac processing,the alkaloid extraction procedure of total alkaloid from konjac was studied.The alkaloid was extracted using acidity alcohol,with berberine as the control,the alkaloid was determined by acid dye Colorimetry.Four factors influenced the extraction were tested in the single factor experiment including ethanol concentration,solid to liquid ratio,extraction time,extraction temperature.The extracting procedure was optimized by orthogonal experiment.The result showed that the main factors on the extraction efficiency were alcohol concentration and the ratio of sample to alcohol.The optimum extraction conditions were:alcohol concentration 75%,the ratio of sample to alcohol 1 ∶15,the temperature of water bath 45℃,extraction time 1.5h.