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2008•Strait Pharmaceutical JournalRequires access

Determining the contents of paeonol in Zhibaidihuang concentrated pill by HPLC

Hong Jing

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Abstract

OBJECTIVE To establish a method for determination of paeonol in Zhibaidihuang concentrated pill.METHODS Using a HPLC method with kromasil KR100-5C18(250×4.6mm)column,the mobile phase was methanol-water(70∶30) at a flow rate of 1.0mL·min-1,the UV detection wavelength was 274nm.RESULTS In the scope of 8.168～163.36μg·mL-1,the contents of paeonol had good linear relationship with their peak area(r=0.9999,n=6)。The average recoveries were 99.12%,RSD=0.52%(n=6).CONCLUSION The results showed that the method was rapid,simple,excellent precision,and could be used for the method of determination.

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OBJECTIVE To establish a method for determination of paeonol in Zhibaidihuang concentrated pill.METHODS Using a HPLC method with kromasil KR100-5C18(250×4.6mm)column,the mobile phase was methanol-water(70∶30) at a flow rate of 1.0mL·min-1,the UV detection wavelength was 274nm.RESULTS In the scope of 8.168～163.36μg·mL-1,the contents of paeonol had good linear relationship with their peak area(r=0.9999,n=6)。The average recoveries were 99.12%,RSD=0.52%(n=6).CONCLUSION The results showed that the method was rapid,simple,excellent precision,and could be used for the method of determination.

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Available abstract

OBJECTIVE To establish a method for determination of paeonol in Zhibaidihuang concentrated pill.METHODS Using a HPLC method with kromasil KR100-5C18(250×4.6mm)column,the mobile phase was methanol-water(70∶30) at a flow rate of 1.0mL·min-1,the UV detection wavelength was 274nm.RESULTS In the scope of 8.168～163.36μg·mL-1,the contents of paeonol had good linear relationship with their peak area(r=0.9999,n=6)。The average recoveries were 99.12%,RSD=0.52%(n=6).CONCLUSION The results showed that the method was rapid,simple,excellent precision,and could be used for the method of determination.

Key concepts: Paeonol, Chromatography, Chemistry, High-performance liquid chromatography, Linear relationship, Content determination, Mathematics, Medicine

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