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2003•Unpublished venueRequires access

Cloning and sequence analysis of HN gene of NDV B_(95)strain

Yanjun Chen

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Abstract

According to published HN protein gene sequence of NDV, we designed and synthesiezed a pair of primers. Genetic RNA of NDV B (95)strain, which was isolated from Brisbane in Australia, was used as template from cDNA synthesis of HN gene. A 1.9 Kb fragment was amplified from B (95) strain and cloned into the PGEM-T-easy vector. The recombinant plasmid was proved to be true by enzyme and PCR. The nucleotide sequence of HN gene was obtained by sanger's sequencing technique. Compared HN gene sequence of B (95)strain with the corresponding sequence of other strains of NDV, the homology of the nucleotide sequence was 95.1%～87%, the homology of the protein sequence was 96.1%～92.1%.

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What this paper is about

According to published HN protein gene sequence of NDV, we designed and synthesiezed a pair of primers. Genetic RNA of NDV B (95)strain, which was isolated from Brisbane in Australia, was used as template from cDNA synthesis of HN gene. A 1.9 Kb fragment was amplified from B (95) strain and cloned into the PGEM-T-easy vector. The recombinant plasmid was proved to be true by enzyme and PCR. The nucleotide sequence of HN gene was obtained by sanger's sequencing technique. Compared HN gene sequence of B (95)strain with the corresponding sequence of other strains of NDV, the homology of the nucleotide sequence was 95.1%～87%, the homology of the protein sequence was 96.1%～92.1%.

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Available abstract

According to published HN protein gene sequence of NDV, we designed and synthesiezed a pair of primers. Genetic RNA of NDV B (95)strain, which was isolated from Brisbane in Australia, was used as template from cDNA synthesis of HN gene. A 1.9 Kb fragment was amplified from B (95) strain and cloned into the PGEM-T-easy vector. The recombinant plasmid was proved to be true by enzyme and PCR. The nucleotide sequence of HN gene was obtained by sanger's sequencing technique. Compared HN gene sequence of B (95)strain with the corresponding sequence of other strains of NDV, the homology of the nucleotide sequence was 95.1%～87%, the homology of the protein sequence was 96.1%～92.1%.

Key concepts: Nucleic acid sequence, Gene, Homology (biology), Biology, Complementary DNA, Sequence analysis, Recombinant DNA, Molecular biology

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