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Cloning and Identifying of P Gene of Newcastle Disease Virus F_(48) E_9 Strain

Cao Dianjun

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Abstract

According to published P protein gene sequence of NDV,we designed and synthesized a pair of primers PU/PL.A 1272bp fragment was amplified from F 48 E 9 strain by means of RT_PCR and cloned into the pMD18_T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the 5' terminal sequence of the cDNA was obtained by sanger's sequencing technique.Compared the obtained gene sequence of F 48 E 9 strain with the corresponding sequence of La Sota strain by DNASIS software,the homology of the nucleotide sequence was 83.7%.As a result,we obtained complete P gene clone of NDV F 48 E 9 strain.

About this research paper

What this paper is about

According to published P protein gene sequence of NDV,we designed and synthesized a pair of primers PU/PL.A 1272bp fragment was amplified from F 48 E 9 strain by means of RT_PCR and cloned into the pMD18_T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the 5' terminal sequence of the cDNA was obtained by sanger's sequencing technique.Compared the obtained gene sequence of F 48 E 9 strain with the corresponding sequence of La Sota strain by DNASIS software,the homology of the nucleotide sequence was 83.7%.As a result,we obtained complete P gene clone of NDV F 48 E 9 strain.

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Available abstract

According to published P protein gene sequence of NDV,we designed and synthesized a pair of primers PU/PL.A 1272bp fragment was amplified from F 48 E 9 strain by means of RT_PCR and cloned into the pMD18_T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the 5' terminal sequence of the cDNA was obtained by sanger's sequencing technique.Compared the obtained gene sequence of F 48 E 9 strain with the corresponding sequence of La Sota strain by DNASIS software,the homology of the nucleotide sequence was 83.7%.As a result,we obtained complete P gene clone of NDV F 48 E 9 strain.

Key concepts: Biology, Gene, Homology (biology), Molecular biology, Cloning (programming), Complementary DNA, Recombinant DNA, Nucleic acid sequence

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Cloning and Identifying of P Gene of Newcastle Disease Virus F_(48) E_9 Strain — Research Paper | ScholarLens