Cloning and sequence analysis of HN gene of a Newcastle disease virus isolate.

The HN gene of Newcastle disease virus isolate(NDV) JZ05 was amplified with reverse transcription-polymerase chain reaction(RT-PCR),its gene cloning was completed,and its nucleotide sequence was determined.Alignment was executed to the HN gene and its amino acids sequence between NDV isolate JZ05 and other 18 NDV isolates by a sequence analysis software(namely DNAssist).The result of sequence determining to the HN gene showed that there were 1716 bp in the HN gene,and in HN protein there were 571 amino acid residues,5 sites of potential carbohydrate binding,13 cysteine residues,no sixth site(538-540 aa).In HN protein of NDV isolate JZ05 single amino acid residue of 6 sites became different as compared with other 18 NDV isolates.Homology was 94% over for the HN gene nucleotide sequence and amino acid sequence between strain JZ05 and 11 strains(ZJ1,TJ03,Jlan-1-00,SBZ02,SCL03,JS02,GD05,SQD04,SQZ04,YG,Taiwan 95),but homology was about 82% for HN gene nucleotide sequence and about 84% for its amino acid sequence between strains JZ05 and 3 vaccine strains(Clone30,LaSota,B1).