Effect of Total Glucoside of Picrorhiza scrophulariflora on Nonenzymatic Glycosylation in Glomerular Mesangial Cells Induced by High Glucose

OBJECTIVE To investigate the effects of TGP on advanced glycation end products( AGEs) and oxidative stress in bovine glomerular mesangial cells cultured in high glucose media and discuss the relationship between intracellular AGEs and oxidative stress. METHODS Bovine glomerular mesangial cells were cultured. Mesangial cells were cultured in high glucose media for 3 weeks to make mesangial cells injury models,and treated with TGP in the meantime. AG and DMSO were treated as control drugs. Then collagen Ⅳ excreted by mesangial cells were detected,and the percentage of cell cycle were observed by flow cytometry technique. The levels of reactive oxygen species(ROS) and mitochondrial membrance potential (MMP) were evaluated by flow cytometric analysis after fluorescent probe DCFH-DA and Rhodamine 123 were loaded. Cellular AGEs were measured by HPLC using a flow injection system. RESULTS TGP significantly decreased the excretion of collagen Ⅳ and cell hypertrophy induced by high glucose,reduced the levels of ROS,inhibited the formation of AGEs and increased MMP in mesangial cells. CONCLUSION The formation of AGEs and oxidative stress accelerate each other in the pathogenesis of diabetic complications,which is called glycoxidation. TGP can protect mesangial cells by inhibiting glycoxidation induced by high glucose.