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2004•Journal of Jilin University ofRequires access

Effects of advanced glycation end products on matrix metalloproteinase-2 activity and expressionin rat mesangial cells

Zhao Zhitao

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Abstract

Objective To investigate the effects of advanced glycation end products (AGEs) on matrix metalloproteinase-2 (MMP-2) activity and expression in rat mesangial cells. Methods Rat mesangial cells were treated with 100 mg·L -1 AGE-modified bovine serum albumin(AGEs group)or native bovine serum albumin(BSA group)for 48 h. Normal mesangial cells without any treatment were as a control (DMEM group). MMP-2 activity and mRNA were analyzed by zymography analysis and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Results Both MMP-2 activity and mRNA expression of mesangial cells in AGEs group were reduced significantly, compared with BSA or DMEM group (P0.01). Conclusion AGEs can reduce the activity and expression of MMP-2 in rat mesangial cells.

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Objective To investigate the effects of advanced glycation end products (AGEs) on matrix metalloproteinase-2 (MMP-2) activity and expression in rat mesangial cells. Methods Rat mesangial cells were treated with 100 mg·L -1 AGE-modified bovine serum albumin(AGEs group)or native bovine serum albumin(BSA group)for 48 h. Normal mesangial cells without any treatment were as a control (DMEM group). MMP-2 activity and mRNA were analyzed by zymography analysis and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Results Both MMP-2 activity and mRNA expression of mesangial cells in AGEs group were reduced significantly, compared with BSA or DMEM group (P0.01). Conclusion AGEs can reduce the activity and expression of MMP-2 in rat mesangial cells.

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Available abstract

Objective To investigate the effects of advanced glycation end products (AGEs) on matrix metalloproteinase-2 (MMP-2) activity and expression in rat mesangial cells. Methods Rat mesangial cells were treated with 100 mg·L -1 AGE-modified bovine serum albumin(AGEs group)or native bovine serum albumin(BSA group)for 48 h. Normal mesangial cells without any treatment were as a control (DMEM group). MMP-2 activity and mRNA were analyzed by zymography analysis and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Results Both MMP-2 activity and mRNA expression of mesangial cells in AGEs group were reduced significantly, compared with BSA or DMEM group (P0.01). Conclusion AGEs can reduce the activity and expression of MMP-2 in rat mesangial cells.

Key concepts: Glycation, Advanced glycation end-product, Mesangial cell, Bovine serum albumin, Chemistry, Matrix metalloproteinase, Messenger RNA, Zymography

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Effects of advanced glycation end products on matrix metalloproteinase-2 activity and expressionin rat mesangial cells — Research Paper | ScholarLens