RAPD Analysis of Popular and Precious Camellia Germplasms
Jun Liu
Abstract
Jun Liu
Abstract
The fingerprints of twenty-three Chinese and foreign Camellia varieties were constructed by RAPD. 136 bands were produced with twenty effectual primers screened from 100 random primers and the genetically polymorphic bands numbered 120 accounting for 88. 2%. The analysis of the genetic similarities showed that the Nei's similarity indexes of various genotypes ranged within 0. 438 6-0. 893 6,averaging at 0. 766 8. Their genetic dendrogram were drawn by UPGMA, which indicated that the twenty-three varieties could be classified into 3 groups. The study results illustrated that RAPD could apply to identifying Camellia varieties and studying genetic polymorphisms of Camellia germplasms.
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The fingerprints of twenty-three Chinese and foreign Camellia varieties were constructed by RAPD. 136 bands were produced with twenty effectual primers screened from 100 random primers and the genetically polymorphic bands numbered 120 accounting for 88. 2%. The analysis of the genetic similarities showed that the Nei's similarity indexes of various genotypes ranged within 0. 438 6-0. 893 6,averaging at 0. 766 8. Their genetic dendrogram were drawn by UPGMA, which indicated that the twenty-three varieties could be classified into 3 groups. The study results illustrated that RAPD could apply to identifying Camellia varieties and studying genetic polymorphisms of Camellia germplasms.
Key concepts: RAPD, UPGMA, Camellia, Dendrogram, Biology, Similarity (geometry), Genetic similarity, Genotype