Mechanism research of allicin inducing apoptosis to cervical cancer cells by upregulation of TRAIL

Objective To observe the growth inhibited effect on Hela and Caski cells induced by allicin,and the expressions of TRAIL,DR4,DR5,Caspase-3,8,9 activity before and after allicin treatments.Furthermore to discuss the mechanism of allicin inducing apoptosis to cervical cancer cells.Methods ①Hela and Caski cells were trained in vitro,to observe the growth inhibitory rates of cells treated by different dose of allicin.②Expressions of TRAIL mRNA before and after allicin treatment were tested by RT-PCR.③Expressions of TRAIL,DR4,DR5 before and after allicin treatment were determined by FCM.④Caspase-3,8,9 activity changes before and after allicin treatment were determined.Results ①Inhibitory rates of Hela and Caski cells after allicin treatments had a time-and-dose-dependent effect.②TRAIL mRNA in Hela and Caski cells ascended markedly after Allicin treatment 12h(P0.05).③TRAIL expression were up-regulated after Allicin treatment 24h.④Caspase-3,8,9 activities were up-regulated obviously after 48h allicin treatment.Conclusion Allicin could inhibit cell proliferation in cervical cancers cell lines of Hela and Caski in vitro.To up-regulate the expression of TRAIL,further more to activate Caspase-3,8,9 is the important mechanism of apoptosis induction by allicin.