In Vitro Selection And Physiological And Biochemical Analyses of Salt-Tolerant Variant For Wheat

The immature embryo and young inflorescence segments of wheat( Triticum aestivum )were used for selecting salt tolerant variant.The genetic stability of callus and their progenies were studied through physiological and biochemical analyses. The results showed that (1) The salt tolerant callus maintained more fresh weight and dry weight even at higher NaCl concerntration than the control callus;(2)The salt tolerant callus maintained higher K +/Na + ratio; (3)Three additional bands of gliadin(B2,B3,B4) were found in the grains of salt tolerant wheat line and B1 band content increased.However B5 band of the control was markedly stronger than that in salt tolerant line; and 4.28 bands of soluble protein appeared in seedling of salt tolerant variant, whereas only 26 bands in CK. 98 kD,75 kD,52 kD,49 kD and 32 kD bands were specific in salt tolerant line, 38 kD,35 kD and 21 kD protein bands only appeared in the control. Characters of salt tolerance in wheat were discussed.