Study on Physiological and Biochemical Characterization in Progenies of Selection of NaCl-tolerant Wheat Mutant by in vitro

Salt-tolerant callus was selected from immature embryo and young inflorescence segments of wheat ( Triticum aestivum ) which seeds were carried by spacecraft,and the salt tolerant stability of their progenies were studied on physiological and biochemical characterization.The results showed that:(1) The relative content of free proline in salt-selected wheat plantlet's progencies was higher than that in unselected ones at higher NaCl concentration soils;(2) The salt-selected lines had higher K~+/Na~+ ratio;(3) Four additional specific electrophoretic bands of gliadin (b2,b3,b5 and b7) were detected and one band (b8) disappeared in F_4 seeds from salt-selected lines;(4) 26 electrophoretic bands of soluble protein were observed in F_4 plants from salt-selected lines,whereas only 23 bands in control lines.Of which 98 kD,75 kD,52 kD,49 kD and (32 kD) bands were specific in salt-tolerant line,38 kD and 35 kD protein bands only appeared in (control) lines.