Determination of the Content and Entrapment Efficiency of Silybin Nanostructured Lipid Carrier

OBJECTIVE To establish a RP-HPLC method for the determination of the content and entrapment efficiency of silybin nano-structured lipid carrier(NLC).METHODS The content of silibin NLC was determined by HPLC.The analytical column was Kromasil C18(4.6 mm×150 mm,5 μm)and the column temperature was ambient.The mobile phase was methanol-0.35 mol·L-1(48:52).The flow rate was l mL·min-1.The detection wavelength was 288 nm.Centrifugation ultrafiltration and sephadex gel filtration methods were employed to isolate free silybin for the entrapment efficiency.RESULTS Silybin was linear in the range of 1～40 mg·L-1(r=0.999 8),the average recovery was 99.57%(RSD=0.52%).The entrapment efficiencies of silybin NLC determinated by sephadex gel filtration and centrifugation ultrafiltration were 84.20% and 96.87%,respectively.CONCLUSION Centrifugation ultrafiltration combined with HPLC is able to determine the entrapment efficiency of silybin NLC efficiently,accurately and conveniently.