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Determination of the Content and Entrapment Efficiency of Decetaxel in Drug Loaded Nano-structured Lipid Carrier

Dongkai Wang

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Abstract

OBJECTIVE:To establish a RP-HPLC method for determination of the content and entrapment efficiency of docetaxel(DTX) in drug loaded nano-structured lipid carrier(NLC).METHODS:The separation was performed with a DiamonsilTM ODS column,and the mobile phase consisted of acetonitrile-water(55∶45) with a flow rate of 1.0 mL·min-1 and UV detection wavelength at 228 nm.Ultracentrifugation and ultrafiltration methods were employed to isolate free decetaxel for the entrapment efficiency determination.RESULTS:Docetaxel had a good linear relation over the range of 0.024～2.4 μg·mL-1(r=0.999 6),the average recovery was 99.29%(RSD=0.37%);The average entrapment efficiency by 2 kinds of methods were more than 96.5%.CONCLUSION:This method is simple,accurate,sensitive and applicable for quality control of this preparation.

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What this paper is about

OBJECTIVE:To establish a RP-HPLC method for determination of the content and entrapment efficiency of docetaxel(DTX) in drug loaded nano-structured lipid carrier(NLC).METHODS:The separation was performed with a DiamonsilTM ODS column,and the mobile phase consisted of acetonitrile-water(55∶45) with a flow rate of 1.0 mL·min-1 and UV detection wavelength at 228 nm.Ultracentrifugation and ultrafiltration methods were employed to isolate free decetaxel for the entrapment efficiency determination.RESULTS:Docetaxel had a good linear relation over the range of 0.024～2.4 μg·mL-1(r=0.999 6),the average recovery was 99.29%(RSD=0.37%);The average entrapment efficiency by 2 kinds of methods were more than 96.5%.CONCLUSION:This method is simple,accurate,sensitive and applicable for quality control of this preparation.

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Available abstract

OBJECTIVE:To establish a RP-HPLC method for determination of the content and entrapment efficiency of docetaxel(DTX) in drug loaded nano-structured lipid carrier(NLC).METHODS:The separation was performed with a DiamonsilTM ODS column,and the mobile phase consisted of acetonitrile-water(55∶45) with a flow rate of 1.0 mL·min-1 and UV detection wavelength at 228 nm.Ultracentrifugation and ultrafiltration methods were employed to isolate free decetaxel for the entrapment efficiency determination.RESULTS:Docetaxel had a good linear relation over the range of 0.024～2.4 μg·mL-1(r=0.999 6),the average recovery was 99.29%(RSD=0.37%);The average entrapment efficiency by 2 kinds of methods were more than 96.5%.CONCLUSION:This method is simple,accurate,sensitive and applicable for quality control of this preparation.

Key concepts: Entrapment, Chromatography, Chemistry, Ultrafiltration (renal), Acetonitrile, Linear range, Content determination, High-performance liquid chromatography

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Determination of the Content and Entrapment Efficiency of Decetaxel in Drug Loaded Nano-structured Lipid Carrier — Research Paper | ScholarLens