Study on Flavone in Buckwheat Determined by Spectrophotometric Method

Flavone in buckwheat is a dietary source.In the world,the largest collection of buckwheat has been conserved in China.For developing and applying functional ingredient in buckwheat,the spectrophotometric method with AlCl3 was investigated to determine the flavone content in buckwheat germplasm,and flavone content of 169 accessions of buckwheat germplasm was evaluated.It indicated that this method possessed characteristics of well-stability,high-accuracy,low-deviation and easy-operating,so it was proved to be suitable for identification and evaluation of buckwheat germplasm.Also,it showed that the average,range and standard deviation of flavone content in tartary buckwheat germplasm was 2.53%,1.94 %-3.11%,0.2436,respectively;and that those of flavone content in common buckwheat germplasm was 0.13%,0.08%-0.21%,0.0273,respectively.The average content of tartary buckwheat germplasm appeared to be 20 times as high as that of common buckwheat germplasm.