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Production of somatic hybrid plants between cabbage and chinese cabbage through protoplast fusion.

Takuro TAGUCHI, Toshiaki Kameya

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Abstract

Protoplasts from leaf mesophyll cells of cabbage (Brassica oleracea L.) and chinese cabbage (B. catnpestris L.) were fused by dextran. Calli exhibiting vigorous growth were selected from the dextran treated protoplasts and plants were regenerated from them after about 3 months of culture. The data of plant and leaf morphology, chromosome number and acid phosphatase isozyme patterns indicate that these plants are interspecific hybrids. This is the first example of production of somatic hybrids between heading type vegetables.

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Protoplasts from leaf mesophyll cells of cabbage (Brassica oleracea L.) and chinese cabbage (B. catnpestris L.) were fused by dextran. Calli exhibiting vigorous growth were selected from the dextran treated protoplasts and plants were regenerated from them after about 3 months of culture. The data of plant and leaf morphology, chromosome number and acid phosphatase isozyme patterns indicate that these plants are interspecific hybrids. This is the first example of production of somatic hybrids between heading type vegetables.

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Available abstract

Protoplasts from leaf mesophyll cells of cabbage (Brassica oleracea L.) and chinese cabbage (B. catnpestris L.) were fused by dextran. Calli exhibiting vigorous growth were selected from the dextran treated protoplasts and plants were regenerated from them after about 3 months of culture. The data of plant and leaf morphology, chromosome number and acid phosphatase isozyme patterns indicate that these plants are interspecific hybrids. This is the first example of production of somatic hybrids between heading type vegetables.

Key concepts: Protoplast, Brassica, Somatic fusion, Brassica oleracea, Biology, Hybrid, Botany, Somatic cell

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Production of somatic hybrid plants between cabbage and chinese cabbage through protoplast fusion. — Research Paper | ScholarLens