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2009•China Medical HeraldRequires access

Quality standard for Hanrebi Capsule

Duan Yanjie

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Abstract

Objective:To establish a quality standard of Hanrebi Capsule.Methods:TLC identifications of Rhizoma Ane-marrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae in Hanrebi Granules were carried out.The content of Paeoniflorin was determined by HPLC.The Dikma Diamonsil C18(4.6 mm×250 mm,5 μm)column was used.The mobile phases consisted of acetonitrile-0.1% and phosphoric acid solution(14∶86), the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm.Results:The characteristic spots of Rhizoma Anemarrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae can be identificated by TLC.The linear ranges of paeoniflorin was at 0.112 4-1.124 0 μg(r=0.999 9), the average recovery was 101.20% with a RSD of 1.71%(n=6).Conclusion:The method is fast, reliable, accurate and can be used in the quality control of Hanrebi Capsule.

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Objective:To establish a quality standard of Hanrebi Capsule.Methods:TLC identifications of Rhizoma Ane-marrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae in Hanrebi Granules were carried out.The content of Paeoniflorin was determined by HPLC.The Dikma Diamonsil C18(4.6 mm×250 mm,5 μm)column was used.The mobile phases consisted of acetonitrile-0.1% and phosphoric acid solution(14∶86), the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm.Results:The characteristic spots of Rhizoma Anemarrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae can be identificated by TLC.The linear ranges of paeoniflorin was at 0.112 4-1.124 0 μg(r=0.999 9), the average recovery was 101.20% with a RSD of 1.71%(n=6).Conclusion:The method is fast, reliable, accurate and can be used in the quality control of Hanrebi Capsule.

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Available abstract

Objective:To establish a quality standard of Hanrebi Capsule.Methods:TLC identifications of Rhizoma Ane-marrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae in Hanrebi Granules were carried out.The content of Paeoniflorin was determined by HPLC.The Dikma Diamonsil C18(4.6 mm×250 mm,5 μm)column was used.The mobile phases consisted of acetonitrile-0.1% and phosphoric acid solution(14∶86), the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm.Results:The characteristic spots of Rhizoma Anemarrhenae, Rhizoma Zingiberis and Radix Glycyrrhizae can be identificated by TLC.The linear ranges of paeoniflorin was at 0.112 4-1.124 0 μg(r=0.999 9), the average recovery was 101.20% with a RSD of 1.71%(n=6).Conclusion:The method is fast, reliable, accurate and can be used in the quality control of Hanrebi Capsule.

Key concepts: Paeoniflorin, Medicine, Capsule, Radix (gastropod), Phosphoric acid, Chromatography, Quality standard, High-performance liquid chromatography

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