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Study on the Quality Standard for Chanhoukang Paste

Pan Jin-huo

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Abstract

Objective To establish the quality standard for Chanhoukang Paste.Methods Rericarpium Citri Reticulatae,Radix Astragali,Radix et Rhizoma Salviae and Radix Glycyrrhizae in this prescription were identified by TLC.The content of paeoniflorin was determined by HPLC.The column of Lichrospher-AQ-C18(4.6 mm×200 mm,5μm)was used.The mobile phase was Acetonitrile-Water(13:87),at the flow of 1.0 ml·min-1.The column temperature was 30℃,and peaks were detected at 230 nm.Results The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The linear range of naringin was 0.536～2.144 μg,r=0.9995,the average recovery was 97.96%,RSD=1.71%(n=6).Conclusion The method is easy,accurate and reproducible.It can be used effectively for the quality control of this preparation.

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Objective To establish the quality standard for Chanhoukang Paste.Methods Rericarpium Citri Reticulatae,Radix Astragali,Radix et Rhizoma Salviae and Radix Glycyrrhizae in this prescription were identified by TLC.The content of paeoniflorin was determined by HPLC.The column of Lichrospher-AQ-C18(4.6 mm×200 mm,5μm)was used.The mobile phase was Acetonitrile-Water(13:87),at the flow of 1.0 ml·min-1.The column temperature was 30℃,and peaks were detected at 230 nm.Results The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The linear range of naringin was 0.536～2.144 μg,r=0.9995,the average recovery was 97.96%,RSD=1.71%(n=6).Conclusion The method is easy,accurate and reproducible.It can be used effectively for the quality control of this preparation.

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Available abstract

Objective To establish the quality standard for Chanhoukang Paste.Methods Rericarpium Citri Reticulatae,Radix Astragali,Radix et Rhizoma Salviae and Radix Glycyrrhizae in this prescription were identified by TLC.The content of paeoniflorin was determined by HPLC.The column of Lichrospher-AQ-C18(4.6 mm×200 mm,5μm)was used.The mobile phase was Acetonitrile-Water(13:87),at the flow of 1.0 ml·min-1.The column temperature was 30℃,and peaks were detected at 230 nm.Results The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The linear range of naringin was 0.536～2.144 μg,r=0.9995,the average recovery was 97.96%,RSD=1.71%(n=6).Conclusion The method is easy,accurate and reproducible.It can be used effectively for the quality control of this preparation.

Key concepts: Paeoniflorin, Chromatography, Radix (gastropod), Naringin, Quality standard, High-performance liquid chromatography, Chemistry, Biology

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