Transgenic of soybean with Bt-cryIA gene mediated by Agrobacterium tumefaciens

The transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(DongNong 50) cotyledon nodes.The transformation of cryIA was confirmed by PCR,RT-PCR and Southern blot.The resistance to soybean pod borer of transgenic plants was detected by bioassay method.114 PPT-resistant plants were obtained and 4,3and 9 positive plants from T0,T1 and T2 generations respectively were tested by PCR.A 1 800bp target fragment was detected by RT-PCR from 3 T1 transgenic plants and the hybridization bands could be detected by Southern-blot assay in two T1 transgenic plants.The gene of cryIA was successfully integrated into the genome of transgenic plants and could be transcripted normally in T1generation.Additionally,the results of bioassay indicated that the transgenic plants displayed high insect-resistance to soybean pod borer.Further investigations on the genetic stability of cryIA in different transgenic lines and generations are to be carried out.