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2012•Journal of Anhui Science and Technology UniversityRequires access

Simultaneous Determination of Seven Kinds of Compounds in Flos Chrysanthemi by HPLC

Li You Meng

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Abstract

To establish a method for determining 7 Kinds of Compounds in Flos Chrysanthemi,acetonitrile-0.1% phosphoric acid aqueous gradient elution,flow rate:0.8mL/min,sampling volume:10μL,detection wavelength:348nm,column temperature 30℃,simultaneous determination of caffeotannic acid,galuteolin,3,5-0-bis caffeoylquinic acid,rutin,baicalin,meletin and acacetin from seven Kinds of Compounds are used.Comparison of HPLC chromatograms has the founding that the peak area ratio of six different chrysanthemum from seven kinds of compound ingredients are very different.The results show that the method is simple,sensitive,direct-viewing,and reveal the differences of chrysanthemum.This provids a reference method for simultaneous determination of multicomponent in chrysanthemum flos and the establishment of fingerprint.

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What this paper is about

To establish a method for determining 7 Kinds of Compounds in Flos Chrysanthemi,acetonitrile-0.1% phosphoric acid aqueous gradient elution,flow rate:0.8mL/min,sampling volume:10μL,detection wavelength:348nm,column temperature 30℃,simultaneous determination of caffeotannic acid,galuteolin,3,5-0-bis caffeoylquinic acid,rutin,baicalin,meletin and acacetin from seven Kinds of Compounds are used.Comparison of HPLC chromatograms has the founding that the peak area ratio of six different chrysanthemum from seven kinds of compound ingredients are very different.The results show that the method is simple,sensitive,direct-viewing,and reveal the differences of chrysanthemum.This provids a reference method for simultaneous determination of multicomponent in chrysanthemum flos and the establishment of fingerprint.

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Available abstract

To establish a method for determining 7 Kinds of Compounds in Flos Chrysanthemi,acetonitrile-0.1% phosphoric acid aqueous gradient elution,flow rate:0.8mL/min,sampling volume:10μL,detection wavelength:348nm,column temperature 30℃,simultaneous determination of caffeotannic acid,galuteolin,3,5-0-bis caffeoylquinic acid,rutin,baicalin,meletin and acacetin from seven Kinds of Compounds are used.Comparison of HPLC chromatograms has the founding that the peak area ratio of six different chrysanthemum from seven kinds of compound ingredients are very different.The results show that the method is simple,sensitive,direct-viewing,and reveal the differences of chrysanthemum.This provids a reference method for simultaneous determination of multicomponent in chrysanthemum flos and the establishment of fingerprint.

Key concepts: Chromatography, Chemistry, Rutin, Phosphoric acid, Gradient elution, Flos, Acacetin, High-performance liquid chromatography

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