Determining the active components alignment of two organic acids on 70% Alcohol Extraction of Flos Chrysanthemi Indici by HPLC

Objective:To develop an HPLC method for determination of two active components alignment of organic acids in Flos Chrysanthemi Indici.Methods:Flos Chrysanthemi Indici from various habitats as carrier and HPLC is used.Results:The chromatographic separation was performed on a Diamonsil C18(4.6×250mm,5μm) eluted with a mobile phase of Acetonitrile-0.5%H3PO4(20∶80).The detection wavelength was set at 326nm and column temperature was set at 30℃.Nice linear relation between the peak area and injected amount exists when the amount is within 0.05944～1.1888μg(r=0.99992) for chlorogenic acid and 0.00384～0.0768μg(r= 0.99997)for caffeic acid.The recoveries of chlorogenic acid is 100.10%(RSD =1.16%) and caffeic acid is 99.74%(RSD=1.03%)(n=5).Conclusions:The method was accurate,repetable and suitable to determining two active components on Flos Chrysanthemi Indici.It can be used for quality control of Flos Chrysanthemi Indici.