2013•Journal of Guangdong Pharmaceutical UniversityRequires access

Study on HPLC fingerprint of Flos Chrysanthem of different sources

Gao Xinka

Abstract

Objective To develop an HPLC method for the fingerprint analysis on flavonoids and phenolic acids from Flos Chrysanthem(HangJu).Methods HPLC was performed with the Kromasil 100-5 C 18 column,and the acetonitrile-0.1% phosphoric acid solution(gradient elution) was employed as a mobile phase.The detection wavelength was 350 nm,and column temperature was 35 ℃.The flow rate was 1.0 mL·min- 1.Results 21 common peaks were identified from 14 batches of Flos Chrysanthem,including 15 flavonoids and 6 phenonic acids.The similarity was evaluated on the Similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine.The similarities of 14 batches were 0.926- 0.998,which indicated that the quality of Flos Chrysanthem was relatively stable.Conclusion The method is accurate and reproducible,which could be used for the quality control of Flos Chrysanthem.

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Key concepts: Flos, Chromatography, Gradient elution, Fingerprint (computing), Phosphoric acid, High-performance liquid chromatography, Chemistry, Similarity (geometry)