Experimental study of vascular endothelial growth factor on inhibiting the apoptosis of vascular endothelial cell

Objective:To evaluate the mechanisms of vascular endothelial growth factor (VEGF) on prevention of restenosis after percutaneous coronary intervention (PCI) by observing the effect of VEGF on the apoptosis of vascular endothelial cell induced by tumor necrosis factor-α(TNF-α).Method:Human umbilical vein endothelial cells (HUVEC) were divided into control group, TNF-α-treated group and TNF-α+VEGF-treated group, the apoptosis of HUVEC was determined by flow cytometry (FCM) and in situ terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL), the expression of Bcl-2 mRNA and Fas mRNA were examined by reverse transcription polymerase chain reaction(RT-PCR).Result:Compared with control group and TNF-α+VEGF-treated group, the apoptosis cells and the expression of apo-1/Fas mRNA were significantly increased of TNF-α-treated group, but the expression of Bcl-2 mRNA was markedly decreased, and VEGF could inhibit the apoptotic effect of TNF-α on HUVEC.Conclusion:VEGF could inhibit the apoptosis of HUVEC induced by TNF-αwhich may correlated with upregulation of Bcl-2 mRNA expression and inhibiting Fas mRNA expression.